Coding

Part:BBa_K2943000:Design

Designed by: Erezyirmiya Yirmiya   Group: iGEM19_TAU_Israel   (2019-09-19)


PAO1 Pyocin Cluster


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 7167
    Illegal PstI site found at 7117
    Illegal PstI site found at 11416
    Illegal PstI site found at 11683
    Illegal PstI site found at 11749
    Illegal PstI site found at 11809
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9367
    Illegal SpeI site found at 7167
    Illegal PstI site found at 7117
    Illegal PstI site found at 11416
    Illegal PstI site found at 11683
    Illegal PstI site found at 11749
    Illegal PstI site found at 11809
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6777
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 7167
    Illegal PstI site found at 7117
    Illegal PstI site found at 11416
    Illegal PstI site found at 11683
    Illegal PstI site found at 11749
    Illegal PstI site found at 11809
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 7167
    Illegal PstI site found at 7117
    Illegal PstI site found at 11416
    Illegal PstI site found at 11683
    Illegal PstI site found at 11749
    Illegal PstI site found at 11809
    Illegal NgoMIV site found at 5672
    Illegal NgoMIV site found at 5760
    Illegal NgoMIV site found at 6849
    Illegal NgoMIV site found at 7099
    Illegal NgoMIV site found at 7399
    Illegal NgoMIV site found at 8074
    Illegal NgoMIV site found at 8305
    Illegal AgeI site found at 5126
    Illegal AgeI site found at 13772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9015
    Illegal BsaI site found at 11469
    Illegal BsaI.rc site found at 7513
    Illegal BsaI.rc site found at 9061
    Illegal SapI site found at 10151
    Illegal SapI.rc site found at 6670


Design Notes

Source

PAO1 genome

References